"This is my greatest tribute to love. Sussanne is and always will be the love of my life for the rest of my life," Hrithik posted on his Facebook page.
"If her smiles are brighter without me, my love for her must accomplish that. Unconditional," added the 39-year-old.
Rumours of a rift between the couple, who dated four years before getting married on December 20, 2000, had been doing the rounds for a few months. Hrithik put the speculation to an end on Friday via an official statement.
"Sussanne has decided to separate from me and end our 17-year relationship. This is a very trying time for the entire family and I request the media and the people to grant us our privacy at this time," he said.
Sussanne, an interior designer and daughter of veteran actor Sanjay Khan, has called the split a matter of "individual choices".
They have two sons together - Hrehaan, seven, and Hridhaan, five.